Composite
Part:BBa_K1480000:Design
Designed by: Roberto Alejandro Valderrama Alfaro Group: iGEM14_UANL_Mty-Mexico (2014-10-08)
Promoter + RBS + TALEN + Terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1334
Illegal NheI site found at 1538
Illegal NheI site found at 1946 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3083
Illegal BamHI site found at 2489 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3023
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1202
Illegal BsaI.rc site found at 854
Illegal SapI.rc site found at 2503
Design Notes
Due the target sequence is based on a eukaryotic sequence, TALEN can bind to an undesirable site.
Source
Synthetic part based on an eukaryotic gene.